RAD51 Antikörper

Produktnummer ABIN4902204

Dieses Anti-RAD51-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von RAD51 in WB und ELISA. Geeignet für Human, Maus, Ratte und Affe.

Kurzübersicht für RAD51 Antikörper (ABIN4902204)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 4H10-G5-H4

Produktdetails anti-RAD51 Antikörper

Verwendungszweck: Rad51 is a protein encoded by the RAD51 gene which is approximately 35 kDa. Rad51 is localised to the nucleus, cytoplasm and mitochondrion matirx. It is involved in the resolution of D-loop structures through synthesis-dependent strand annealing, DNA double-strand break repair, ATM pathway and the BRCA1 pathway. It is known to be involved in the homologous strand exchange, a key step in DNA repair through homologous recombination and repair of DNA. It catalyses the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Rad51 is highly expressed in the testis and thymus and less expressed in the small intestine, placenta, colon, pancreas and ovary. Mutations in the RAD51 gene result in breast cancer and Fanconi anemia, a disorder affecting all bone marrow elements. STJ99103 was developed from clone 4H10-G5-H4. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and detects endogenous levels of Rad51 and does not cross-react with related proteins.

Spezifität: This antibody detects endogenous levels of Rad51 and does not cross-react with related proteins.

Produktmerkmale: Mouse Monoclonal to Rad51.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Recombinant peptide derived from human Rad51.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB 1:500-2000
ELISA 1:10000-20000

Kommentare:

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C, and avoid repeat freeze-thaw cycles.

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: Rad51

Molekulargewicht: 37kDa

Gen-ID: 5888

UniProt: Q06609

Pathways: DNA Reparatur