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CD3 Antikörper

CD3 Reaktivität: Human, Maus FACS, IP, IHC, IHC (fro), WB, ICC Wirt: Maus Monoclonal APA1-1 unconjugated

Produktnummer ABIN490154

Datenblatt (PDF)

Target Alle CD3 Antikörper anzeigen

CD3
Reaktivität
211

105

33

20

17

10

8

8

7

7

7

3

3

2

2
Human, Maus
Wirt
255

54

20

13

9

1
Maus
Passende Sekundärantikörper
Klonalität
341

6

1
Monoklonal
Konjugat
102

43

35

26

25

13

10

8

8

8

6

5

5

5

5

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD3 Antikörper ist unkonjugiert
Applikation
304

93

62

60

46

43

43

23

17

10

9

8

7

7

6

4

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunocytochemistry (ICC)

Spezifität

The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Aufreinigung

Protein A purified

Immunogen

Purified human CD3 proteins isolated from thymus.

Type of Immunogen: Purified protein

Klon

APA1-1

Isotyp

IgG1

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CD3 Reaktivität: Human FACS Wirt: Maus Monoclonal MEM-57 APC

Applikationshinweise

Approved: Flo, ICC, IHC, IHC-Fr, IP, WB

Usage: Flow Cytometry: Positive control - Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Sample preparation:. At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1 % BSA for 20 min. on ice. Wash

Kommentare

Target Species of Antibody: Human

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

Lot specific

Buffer

PBS, 15 mM sodium azide,  pH 7.4

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

avoid freeze thaw cycles.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

CD3

Andere Bezeichnung

CD3 (CD3 Produkte)

Pathways

T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway