Cyclin D1 Antikörper (AA 1-295) (PE)

Produktnummer ABIN4888512

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cyclin D1 in ICFC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Non-Human Primate.

Kurzübersicht für Cyclin D1 Antikörper (AA 1-295) (PE) (ABIN4888512)

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Maus, Ratte, Non-Human Primate

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin D1 Antikörper ist konjugiert mit PE

Applikation: Intracellular Flow Cytometry (ICFC)

Klon: DCS-6

Produktdetails anti-Cyclin D1 Antikörper (PE)

Bindungsspezifität: AA 1-295

Verwendungszweck: Anti-Cyclin D1 PE

Spezifität: The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

Keine Kreuzreaktivität: Cat, Schaf

Kreuzreaktivität (Details): Human, Non-Human Primates, Mouse, Rat

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: recombinant human cyclin D1 (amino acids 1-295)

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1 μg/mL. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1

Hintergrund: Cyclin D1,Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.,CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

Gen-ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling