XPO1 Antikörper (AA 966-1071)
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- Target Alle XPO1 Antikörper anzeigen
- XPO1 (Exportin 1 (XPO1))
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Bindungsspezifität
- AA 966-1071
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser XPO1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Verwendungszweck
- Anti-CRM1/XPO1 Antibody Picoband®
- Kreuzreaktivität (Details)
- No cross-reactivity with other proteins
- Produktmerkmale
- Anti-CRM1/XPO1 Antibody Picoband® (ABIN4886771). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Aufreinigung
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.
- Isotyp
- IgG
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- Applikationshinweise
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x106 cells1. Dong, X., Biswas, A., Suel, K. E., Jackson, L. K., Martinez, R., Gu, H., Chook, Y. M. Structural basis for leucine-rich nuclear export signal recognition by CRM1. Nature 458: 1136-1141, 2009. Note: Erratum: Nature 461: 550 only, 2009. 2. Fornerod, M., van Baal, S., Valentine, V., Shapiro, D. N., Grosveld, G. Chromosomal localization of genes encoding CAN/Nup214-interacting proteins--human CRM1 localizes to 2p16, whereas Nup88 localizes to 17p13 and is physically linked to SF2p32. Genomics 42: 538-540, 1997. 3. Kudo, N., Khochbin, S., Nishi, K., Kitano, K., Yanagida, M., Yoshida, M., Horinouchi, S. Molecular cloning and cell cycle-dependent expression of mammalian CRM1, a protein involved in nuclear export of proteins. J. Biol. Chem. 272: 29742-29751, 1997. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Target
- XPO1 (Exportin 1 (XPO1))
- Andere Bezeichnung
- XPO1 (XPO1 Produkte)
- Hintergrund
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Synonyms: Exportin-1,Exp1,Chromosome region maintenance 1 protein homolog,XPO1,CRM1,
Tissue Specificity: Expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. Not expressed in the kidney. .
Background: Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.
- Molekulargewicht
- 123 kDa
- Gen-ID
- 7514
- UniProt
- O14980
- Pathways
- M Phase
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