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Lactate Dehydrogenase A Antikörper (AA 2-106)

Der Kaninchen Polyklonal Anti-Lactate Dehydrogenase A-Antikörper wurde für WB, IHC, IF, ICC und FACS validiert. Er ist geeignet, Lactate Dehydrogenase A in Proben von Human, Maus und Ratte zu detektieren.
Produktnummer ABIN4886657

Kurzübersicht für Lactate Dehydrogenase A Antikörper (AA 2-106) (ABIN4886657)

Target

Alle Lactate Dehydrogenase A (LDHA) Antikörper anzeigen
Lactate Dehydrogenase A (LDHA)

Reaktivität

  • 85
  • 39
  • 30
  • 11
  • 7
  • 6
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 101
  • 21
  • 6
  • 5
Kaninchen

Klonalität

  • 108
  • 23
Polyklonal

Konjugat

  • 65
  • 21
  • 16
  • 9
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
Dieser Lactate Dehydrogenase A Antikörper ist unkonjugiert

Applikation

  • 97
  • 57
  • 46
  • 35
  • 31
  • 26
  • 9
  • 9
  • 6
  • 4
  • 4
  • 4
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 20
    • 9
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-106

    Verwendungszweck

    Anti-LDHA Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-LDHA Antibody Picoband® (ABIN4886657). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E. coli-derived human LDHA recombinant protein (Position: A2-R106). Human LDHA shares 94.3% amino acid (aa) sequence identity with both mouse and rat LDHA.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Chung FZ, Tsujibo H, Bhattacharyya U, Sharief FS, Li SS (November 1985). "Genomic organization of human lactate dehydrogenase-A gene". Biochem. J. 231 (3): 537-41. 2. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925-7.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    Lactate Dehydrogenase A (LDHA)

    Andere Bezeichnung

    LDHA

    Hintergrund

    Synonyms: L-lactate dehydrogenase A chain,LDH-A,1.1.1.27,Cell proliferation-inducing gene 19 protein,LDH muscle subunit,LDH-M,Renal carcinoma antigen NY-REN-59,LDHA,PIG19,

    Tissue Specificity: Low levels in brain. Very weak expression found in heart and muscle. .

    Background: Lactate dehydrogenase A, also known as LDHA, is an enzyme which in humans is encoded by the LDHA gene. The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

    Molekulargewicht

    37 kDa

    Gen-ID

    3939

    UniProt

    P00338

    Pathways

    Warburg Effekt
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