KDM5B Antikörper (Middle Region)

Produktnummer ABIN4886648

Der Kaninchen-Polyklonal Anti-KDM5B-Antikörper wurde für WB, IHC, FACS, IF und ICC validiert. Er ist geeignet zum Nachweis von KDM5B in Proben aus Human, Maus, Ratte und Affe. }

Kurzübersicht für KDM5B Antikörper (Middle Region) (ABIN4886648)

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KDM5B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-KDM5B Antikörper

Bindungsspezifität: AA 641-685, Middle Region

Verwendungszweck: Anti-KDM5B/PLU1/Jarid1B Antibody

Sequenz: DVLDVVVAST VQKDMAIMIE DEKALRETVR KLGVIDSERM DFE

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-KDM5B/PLU1/Jarid1B Antibody (ABIN4886648). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human KDM5B, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu KDM5B

Target: KDM5B (Lysine (K)-Specific Demethylase 5B (KDM5B))

Andere Bezeichnung: KDM5B

Hintergrund:

Synonyms: Lysine-specific demethylase 5B,1.14.11.-,Cancer/testis antigen 31,CT31,Histone demethylase JARID1B,Jumonji/ARID domain-containing protein 1B,PLU-1,Retinoblastoma-binding protein 2 homolog 1,RBP2-H1,KDM5B,JARID1B, PLU1, RBBP2H1,

Tissue Specificity: Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level). .

Background: Lysine-specific demethylase 5B, also known as histone demethylase JARID1B, is a demethylase enzyme that in humans is encoded by the KDM5B gene. This gene encodes a lysine-specific histone demethylase that belongs to the jumonji/ARID domain-containing family of histone demethylases. The encoded protein is capable of demethylating tri-, di- and monomethylated lysine 4 of histone H3. This protein plays a role in the transcriptional repression or certain tumor suppressor genes and is upregulated in certain cancer cells. This protein may also play a role in genome stability and DNA repair. Alternate splicing resultsi n multiple transcript variants.

Molekulargewicht: 180 kDa

Gen-ID: 10765

Pathways: Warburg Effekt