Hexokinase 2 Antikörper (Middle Region)

Produktnummer ABIN4886617

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Hexokinase 2 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Hexokinase 2 Antikörper (Middle Region) (ABIN4886617)

Target: Hexokinase 2 (HK2)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexokinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Hexokinase 2 Antikörper

Bindungsspezifität: AA 460-497, Middle Region

Verwendungszweck: Anti-Hexokinase II/HK2 Antibody Picoband®

Sequenz: AYRLADQHRA RQKTLEHLQL SHDQLLEVKR RMKVEME

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Hexokinase II/HK2 Antibody Picoband® (ABIN4886617). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Hexokinase II, different from the related mouse and rat sequences by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: HK2 hexokinase 2". 2. Printz RL, Osawa H, Ardehali H, Koch S, Granner DK (Feb 1997). "Hexokinase II gene: structure, regulation and promoter organization". Biochemical Society Transactions. 25 (1): 107-12. 3. Peng Q, Zhou J, Zhou Q, Pan F, Zhong D, Liang H (2009). "Silencing hexokinase II gene sensitizes human colon cancer cells to 5-fluorouracil". Hepato-Gastroenterology. 56 (90): 355-60.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Hexokinase 2

Target: Hexokinase 2 (HK2)

Andere Bezeichnung: HK2

Hintergrund:

Synonyms: Hexokinase-2,2.7.1.1,Hexokinase type II,HK II,Muscle form hexokinase,HK2,

Tissue Specificity: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Background: Hexokinase 2, also known as HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Molekulargewicht: 102 kDa

Gen-ID: 3099

UniProt: P52789

Pathways: PI3K-Akt Signalweg, Carbohydrate Homeostasis, Warburg Effekt