Hexokinase 2 Antikörper (Middle Region)

Produktnummer ABIN4886617

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch Hexokinase 2 in WB, IHC und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Hexokinase 2 Antikörper (Middle Region) (ABIN4886617)

Target: Hexokinase 2 (HK2)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexokinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-Hexokinase 2 Antikörper

Bindungsspezifität: AA 460-497, Middle Region

Verwendungszweck: Anti-Hexokinase II/HK2 Antibody

Sequenz: AYRLADQHRA RQKTLEHLQL SHDQLLEVKR RMKVEME

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Hexokinase II/HK2 Antibody (ABIN4886617). Tested in WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Hexokinase II, different from the related mouse and rat sequences by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Hexokinase 2

Target: Hexokinase 2 (HK2)

Andere Bezeichnung: HK2

Hintergrund:

Synonyms: Hexokinase-2,2.7.1.1,Hexokinase type II,HK II,Muscle form hexokinase,HK2,

Tissue Specificity: Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle.

Background: Hexokinase 2, also known as HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Molekulargewicht: 102 kDa

Gen-ID: 3099

UniProt: P52789

Pathways: PI3K-Akt Signalweg, Carbohydrate Homeostasis, Warburg Effekt