Adenosine A2a Receptor Antikörper

Produktnummer ABIN487435

Dieser Maus Monoklonal Antikörper erkennt spezifisch Adenosine A2a Receptor in WB, FACS und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Kaninchen und Meerschweinchen.

Kurzübersicht für Adenosine A2a Receptor Antikörper (ABIN487435)

Target: Adenosine A2a Receptor (ADORA2A)

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Adenosine A2a Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: N-n

Produktdetails anti-Adenosine A2a Receptor Antikörper

Spezifität: This antibody recognizes Human Adenosine Receptor A2A at ~43 kDa. Does not recognize Adenosine Receptor A2A in knockout mice.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Guinea Pig and Rabbit.
Species reactivity (tested):Human.

Produktmerkmale: Synonyms: ADORA2A, ADORA2

Aufreinigung: Protein G Chromatography

Immunogen: 6His-tagged full-length Human recombinant A2A receptor. Genename: ADORA2ARemarks: Epitope mapped to SQPLPGER in the third intracellular loop.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000-1/5000. Flow Cytometry. Immunohistochemistry (Paraffin Sections): 5 μg/mL. Immunohistochemistry (4 % Paraformaldehyde): 1 μg/mL. This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.01 M Tris-Glycine, pH 7.4, 0.11 M sodium chloride, 0.05 % sodium azide.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Andere Bezeichnung: Adenosine Receptor A2a

Hintergrund: The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.Synonyms: ADORA2, ADORA2A

Gen-ID: 135

UniProt: P29274

Pathways: Neurotrophin Signalübertragung, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints