CD3 Antikörper

Produktnummer ABIN487434

Produktdetails anti-CD3 Antikörper

Target: CD3

Reaktivität: Maus

Wirt: Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunofluorescence (IF)

Spezifität: This antibody reacts with Mouse CD3 (epsilon subunit). It is commonly used as a phenotypic marker for Mouse T cells.

Kreuzreaktivität (Details): Species reactivity (tested):Mouse.

Produktmerkmale: Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4

Aufreinigung: Protein A affinity chromatography

Immunogen: Mouse BM10-37 cytotoxic T lymphocytes

Klon: 145-2C11

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Flow cytometry: 1 μg/mL (million cells). Immunoprecipitation: 1-2 μg/100-500 μg protein in 1 mLcell lysate. Immunohistochemistry on frozen sections. Immunoflourescence. Functional application: Induction of T cell activation, proliferation or apoptosis (dependingon conditions), in vivo T cell depletion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CD3

Target: CD3

Andere Bezeichnung: CD3 (CD3 Produkte)

Hintergrund: CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gen-ID: 12501

UniProt: P22646

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway