Nitrotyrosine Antikörper

Produktnummer ABIN479556

Dieses Anti-Nitrotyrosine-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Nitrotyrosine in WB, IP und ICC. Geeignet für .

Kurzübersicht für Nitrotyrosine Antikörper (ABIN479556)

Target: Nitrotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nitrotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-Nitrotyrosine Antikörper

Spezifität: Recognizes nitrated proteins from all species.

Aufreinigung: Protein G purified

Immunogen: Purified, nitrated Keyhole Limpet Hemocyanin (KLH).

Type of Immunogen: Hapten

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Approved: ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Immunoprecipitation: To be determined by the researcher. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for nitrated proteins in peroxynitrite-treated A431 cells fixed with 95 % ethanol/5 % acetic acid.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Nitrotyrosine

Target: Nitrotyrosine

Substanzklasse: Chemical