KIT Ligand Antikörper (Biotin)

Produktnummer ABIN465802

Dieser Anti-KIT Ligand-Antikörper ist ein Kaninchen-Polyklonal-Antikörper zum Nachweis von KIT Ligand in WB, ELISA und Neut. Geeignet für Maus.

Kurzübersicht für KIT Ligand Antikörper (Biotin) (ABIN465802)

Target: KIT Ligand (KITLG)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Ligand Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-KIT Ligand Antikörper (Biotin)

Spezifität: Mouse SCF

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine SCF.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mSCF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mSCF. To detect mSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine SCF (LS-C104783) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mSCF. Western Blot: To detect mSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu KIT Ligand

Target: KIT Ligand (KITLG)

Andere Bezeichnung: KITLG / SCF

Hintergrund: Name/Gene ID: KITLG

Synonyms: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF

Gen-ID: 4254

UniProt: P21583

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung