IL-5 Antikörper
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- Target Alle IL-5 (IL5) Antikörper anzeigen
- IL-5 (IL5) (Interleukin 5 (IL5))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser IL-5 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Spezifität
- Human IL-5
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human IL-5.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL5 Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-5 (5 ng/mL), a concentration of 0.05-0.15 μg/mL of this antibody is required. ELISA: To detect Human IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (LS-C104567) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- IL-5 (IL5) (Interleukin 5 (IL5))
- Andere Bezeichnung
- IL5 (IL5 Produkte)
- Synonyme
- EDF antikoerper, IL-5 antikoerper, TRF antikoerper, Il-5 antikoerper, IL5 antikoerper, interleukin 5 antikoerper, IL5 antikoerper, Il5 antikoerper
- Hintergrund
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Name/Gene ID: IL5
Family: Hormone
Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF - Gen-ID
- 3567
- UniProt
- P05113
- Pathways
- JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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