Midkine Antikörper (Biotin)

Produktnummer ABIN465384

Der Kaninchen Polyklonal anti-Midkine Antikörper wird verwendet zum Nachweis von Midkine in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für Midkine Antikörper (Biotin) (ABIN465384)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Midkine Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Midkine Antikörper (Biotin)

Spezifität: Human Midkine

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Midkine.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hMidkine by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMidkine. To detect hMidkine by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Midkine (LS-B8221) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Andere Bezeichnung: MDK / Midkine

Hintergrund: Name/Gene ID: MDK

Synonyms: MDK, ARAP, MK, MK1, NEGF2, Midkine, Retinoic acid inducible factor

Gen-ID: 4192

UniProt: P21741

Pathways: RTK Signalweg, M Phase, Skeletal Muscle Fiber Development