VEGFA Antikörper (Biotin)
Kurzübersicht für VEGFA Antikörper (Biotin) (ABIN465276)
Target
Alle VEGFA Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- Human VEGF
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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E.coli derived recombinant Human VEGF.
Type of Immunogen: Cell extract
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Applikationshinweise
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Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hVEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hVEGF. To detect hVEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human VEGF (LS-C104614) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Sterile PBS containing 0.1 % BSA
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Konzentration
- Lot specific
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Buffer
- Lyophilized from PBS, pH 7.2
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Handhabung
- Avoid freeze/thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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Andere Bezeichnung
- VEGFA / VEGF
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Hintergrund
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Name/Gene ID: VEGFA
Family: PDGF
Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1 -
Gen-ID
- 7422
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UniProt
- P15692
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Pathways
- RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
Target
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