HSPD1 Antikörper

Produktnummer ABIN462364

Dieses Maus Monoklonal-Antikörper erkennt spezifisch HSPD1 in WB, IHC, IP und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn, Affe, Hamster, Hund, Rind (Kuh), Kaninchen, Schwein, Meerschweinchen, Pflanzen und C. elegans.

Kurzübersicht für HSPD1 Antikörper (ABIN462364)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Hamster, Hund, Rind (Kuh), Kaninchen, Schwein, Meerschweinchen, Pflanzen, C. elegans

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSPD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: LK-2

Produktdetails anti-HSPD1 Antikörper

Marke: IHC-plus™

Spezifität: Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).

Aufreinigung: Protein G purified

Immunogen: Human HSP60 produced through recombinant DNA methods in E. coli.

Type of Immunogen: Recombinant protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (10 μg/mL), IP (10 μg/mL), WB (0.25 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 mM PMSF, 50 % glycerol

Konservierungsmittel: PMSF

Handhabung: avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Details zu HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Andere Bezeichnung: HSPD1 / HSP60

Hintergrund: Name/Gene ID: HSPD1

Synonyms: HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL

Gen-ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity