alpha 2 Macroglobulin Antikörper

Produktnummer ABIN458636

Der Ziege Polyklonal Anti-alpha 2 Macroglobulin-Antikörper wurde für IEP validiert. Er ist geeignet, alpha 2 Macroglobulin in Proben von Ratte zu detektieren.

Kurzübersicht für alpha 2 Macroglobulin Antikörper (ABIN458636)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser alpha 2 Macroglobulin Antikörper ist unkonjugiert

Applikation: Immunoelectrophoresis (IEP)

Produktdetails anti-alpha 2 Macroglobulin Antikörper

Spezifität: The antiserum does not cross-react with any other component of rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction has been observed with mouse.

Produktmerkmale: Precipitating polyclonal goat antiserum to rat alpha-2 macroglobulin

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Alpha-2 macroglobulin(clotting factor I) is the major protein in the alpha-2 region of rat serum after separation by immunoelectrophoresis. The concentration in normal plasma is about 2.5 mg/ml. It is a large polymer and a strong antigen. It forms about one third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gelfiltration and preparative starch block electrophoresis. In the purification the activation of kallikrein is avoided, since it forms an irreversible bond with alpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and single or double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of alpha-2 macroglobulin in rat plasma or other body fluids or to determine its concentration. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays, but this does exclude such use if proper controls are included.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:8 when tested against pooled normal rat plasma in agar-block immunodiffusion titration

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Details zu alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Andere Bezeichnung: alpha-2 Macroglobulin

Hintergrund: The reactivity of the antiserum is restricted to rat alpha-2 macroglobulin. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against normal rat plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-2 macroglobulin. No reaction is obtained with any other plasma protein components or serum

Pathways: Lipid Metabolism