CFP Antikörper (FITC)

Produktnummer ABIN458013

Produktdetails anti-CFP Antikörper (FITC)

Target: CFP (Complement Factor P (CFP))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CFP Antikörper ist konjugiert mit FITC

Applikation: ELISA, Immunofluorescence (IF)

Spezifität: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to human properdin

Aufreinigung: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by saltprecipitation and purification of the IgG fraction by DEAE-chromatography.

Immunogen: Properdin is a factor of the alternative complement activation pathway. It has a molecular weight of 222,000 and consists of four peptide chains. Properdin is stabilizing the C3 convertase C3bBb. The mean concentration in plasma is 20 mg/ml. The antigen has been isolated form pooled human plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: As reagent for the direct detection of properdin in human cells, tissues and body fluids in immuno-fluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:60.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: IgG protein concentration 10.7 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.3. No foreign proteins added.

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Konservierungsmittel: Without preservative

Lagerung: 4 °C

Details zu CFP

Target: CFP (Complement Factor P (CFP))

Andere Bezeichnung: Properdin (CFP Produkte)

Synonyme: BFD antikoerper, PFC antikoerper, PFD antikoerper, PROPERDIN antikoerper, BCFG antikoerper, Pfc antikoerper, Properdin antikoerper, complement factor properdin antikoerper, CFP antikoerper, Cfp antikoerper

Hintergrund: Tested in immunoelectrophoresis against human serum a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma

Pathways: Komplementsystem