EGFR Antikörper (Internal Region)

Produktnummer ABIN457474

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch EGFR in WB, ELISA, IHC, IF, IHC (p), IP und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Huhn.

Kurzübersicht für EGFR Antikörper (Internal Region) (ABIN457474)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Produktdetails anti-EGFR Antikörper

Bindungsspezifität: Internal Region

Marke: IHC-plus™

Spezifität: Recognizes human Epidermal Growth Factor Receptor.

Aufreinigung: Protein G purified

Immunogen: Recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region.

Type of Immunogen: Recombinant protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, IF, IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.15 M PBS,  pH 7.4. No preservatives added.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR

Hintergrund: Name/Gene ID: EGFR
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: EGFR, EGFf receptor, ERBB, ERBB1, MENA, PIG61, Proto-oncogene c-ErbB-1, V-erb-b homolog, EGF-R, HER1

Gen-ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine