Integrin beta 2 Antikörper

Produktnummer ABIN457430

Der Ratte Monoklonal Anti-Integrin beta 2-Antikörper wurde für FACS, WB, IP, IHC (fro) und IHC (p) validiert. Er ist geeignet, Integrin beta 2 in Proben von Maus zu detektieren. Es sind 8+ Publikationen verfügbar.

Kurzübersicht für Integrin beta 2 Antikörper (ABIN457430)

Target: Integrin beta 2 (ITGB2)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: M18-2

Produktdetails anti-Integrin beta 2 Antikörper

Verwendungszweck: Anti-Ms CD18 Purified

Spezifität: The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit, beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

Keine Kreuzreaktivität: Hund

Kreuzreaktivität (Details): Mouse

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Murine cytotoxic T cell glycoproteins

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Integrin beta 2 Antikörper (ABIN457430)

Cullere, Lauterbach, Tsuboi, Mayadas: "Neutrophil-selective CD18 silencing using RNA interference in vivo." in: Blood, Vol. 111, Issue 7, pp. 3591-8, (2008) (PubMed).

Varga, Balkow, Wild, Stadtbaeumer, Krummen, Rothoeft, Higuchi, Beissert, Wethmar, Scharffetter-Kochanek, Vestweber, Grabbe: "Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation." in: Blood, Vol. 109, Issue 2, pp. 661-9, (2007) (PubMed).

Watts, Beurskens, Martin-Padura, Ballantyne, Klickstein, Brenner, Lee: "Manifestations of inflammatory arthritis are critically dependent on LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 6, pp. 3668-75, (2005) (PubMed).

Barlow, Langston, Matthews, Chidlow, Kevil: "CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes." in: The American journal of pathology, Vol. 165, Issue 6, pp. 1849-52, (2004) (PubMed).

Sakurai, Taguchi, Anand, Ambati, Gragoudas, Miller, Adamis, Ambati: "Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization." in: Investigative ophthalmology & visual science, Vol. 44, Issue 6, pp. 2743-9, (2003) (PubMed).

Abraham, Miller: "Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5193-201, (2001) (PubMed).

Avni, Pur, Yefenof, Baniyash: "Complement receptor 3 of macrophages is associated with galectin-1-like protein." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 6151-8, (1998) (PubMed).

Sanchez-Madrid, Simon, Thompson, Springer: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, (1983) (PubMed).

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: CD18

Hintergrund: Integrin subunit beta 2,CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.,Integrin beta2, Complement receptor C3 subunit beta, ITGB2

Gen-ID: 16414

UniProt: P11835

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation