GFAP Antikörper (Glial Fibrillary Acidic Protein)

Details for Product anti-GFAP Antibody No. ABIN457420, Anbieter: Anmelden zum Anzeigen
Antigen
  • GFAP
  • AI836096
  • cb345
  • etID36982.3
  • gfapl
  • wu:fb34h11
  • wu:fk42c12
  • xx:af506734
  • zgc:110485
  • glial fibrillary acidic protein
  • GFAP
  • LOC100136168
  • gfap
  • Gfap
Reaktivität
Human, Schwein, Ratte (Rattus)
732
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102
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32
25
24
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21
12
7
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Wirt
Maus
464
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23
4
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2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser GFAP Antikörper ist unkonjugiert
34
24
24
17
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11
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9
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5
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Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
626
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140
89
72
53
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9
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3
1
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Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Immunogen Porcine spinal cord
Klon GA-5
Isotyp IgG1
Spezifität The antibody GA-5 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves.
The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
Reinigung Purified from cell culture supernatant by protein-A affinity chromatography.
Reinheit > 95 % (by SDS-PAGE)
Andere Bezeichnung GFAP (GFAP Antibody Abstract)
Hintergrund Glial Fibrillary Acidic Protein (GFAP) was discovered by Bignami et al. (1972) as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10 nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin. GFAP is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. It is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. Although its function is not fully understood, GFAP protein is probably involved in controlling the shape and movement of astrocytes. The protein probably also plays a significant role in the interactions of astrocytes with other cells, which are required for the formation and maintenance of the insulating layer (myelin) that covers nerve cells. Additionally, GFAP protein may assist in maintaining the protective barrier that allows only certain substances to pass between blood vessels and the brain (blood-brain barrier).In adults, GFAP levels increase as a result of the proliferation of astrocytes that occurs in a response to a variety of physical, chemical and etiological insults, including Alzheimer’,s disease, epilepsy and multiple sclerosis.Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells and for distinguishing of neoplasms of astrocytic origin from other neoplasms in the central nervous system. Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (Brenner et al., 2001). All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Produkt verwendet in: Joardar, Sen, Das: "Docosahexaenoic acid facilitates cell maturation and beta-adrenergic transmission in astrocytes." in: Journal of lipid research, Vol. 47, Issue 3, pp. 571-81, 2006 (PubMed).

Guillemin, Wang, Brew: "Quinolinic acid selectively induces apoptosis of human astrocytes: potential role in AIDS dementia complex." in: Journal of neuroinflammation, Vol. 2, pp. 16, 2005 (PubMed).

Rungger-Brändle, Dosso, Leuenberger: "Glial reactivity, an early feature of diabetic retinopathy." in: Investigative ophthalmology & visual science, Vol. 41, Issue 7, pp. 1971-80, 2000 (PubMed).

Perng, Cairns, van den IJssel, Prescott, Hutcheson, Quinlan: "Intermediate filament interactions can be altered by HSP27 and alphaB-crystallin." in: Journal of cell science, Vol. 112 ( Pt 13), pp. 2099-112, 1999 (PubMed).

Allgemeine Veröffentlichungen Brenner, Johnson, Boespflug-Tanguy, Rodriguez, Goldman, Messing: "Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease." in: Nature genetics, Vol. 27, Issue 1, pp. 117-20, 2001 (PubMed).

Bignami, Eng, Dahl, Uyeda: "Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence." in: Brain research, Vol. 43, Issue 2, pp. 429-35, 1972 (PubMed).

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