beta Catenin Antikörper

Produktnummer ABIN457419

Dieses Anti-beta Catenin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von beta Catenin in WB, IP, ICC und ICFC. Geeignet für Human, Maus und Hamster.

Kurzübersicht für beta Catenin Antikörper (ABIN457419)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reaktivität: Human, Maus, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser beta Catenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Klon: EM-22

Produktdetails anti-beta Catenin Antikörper

Verwendungszweck: Anti-beta-Catenin Purified

Spezifität: The mouse monoclonal antibody EM-22 reacts with C-terminal part of beta-catenin (intracellular antigen), an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription.

Kreuzreaktivität (Details): Human, Mouse, Hamster

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant human beta-catenin

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line.
Western blotting: Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line, KW1 murine cell line, C57MG murine cell line, 3T3 murine fibroblast cell line.
Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Andere Bezeichnung: beta-Catenin

Hintergrund: Catenin beta 1,Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.,CTNNB1, EVR7, MRD19

Gen-ID: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism