SOD3 Antikörper

Produktnummer ABIN453955

Dieser Maus Monoklonal Antikörper detektiert spezifisch SOD3 in WB, IF, IHC (fro) und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für SOD3 Antikörper (ABIN453955)

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Klon: 4G11G6

Produktdetails anti-SOD3 Antikörper

Spezifität: This antibody detects EC-SOD at ~35 kDa. Does not cross react with other SODs.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Rat, Mouse.

Produktmerkmale: Synonyms: SOD-3, EC-SOD, Extracellular superoxide dismutase [Cu-Zn]

Aufreinigung: Protein G Chromatography

Immunogen: Human EC-SOD purified from aortas

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000.1 μg/mL was sufficient for detection of EC-SOD in 20 μg of Human cartillage lysate bycolorimetric Immunoblot using Goat anti-Mouse IgG (HRP) as the secondary antibodyELISA (7). Immunocytochemistry: 1/100. Immunohistochemistry on Frozen Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Haltbarkeit: 12 months

Details zu SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Andere Bezeichnung: Superoxide Dismutase 3 / SOD3

Hintergrund: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: EC-SOD, Extracellular superoxide dismutase [Cu-Zn], SOD-3

Gen-ID: 6649

UniProt: P08294