CRYAA Antikörper

Produktnummer ABIN453938

Der Maus Monoklonal anti-CRYAA Antikörper wird verwendet zum Nachweis von CRYAA in Proben von Human, Maus, Ratte und Rind (Kuh). Er wurde validiert für WB und EIA.

Kurzübersicht für CRYAA Antikörper (ABIN453938)

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CRYAA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: 1H3-B8

Produktdetails anti-CRYAA Antikörper

Spezifität: This antibody recognizes Alpha A Crystallin at ~20 kDa. Detects alpha Crystallin complex (containing Alpha A and Alpha B subunits). Does not cross react with Alpha B Crystallin, Beta-L Crystallin, Beta-H Crystallin, Gamma Crystallin, Hsp25, Hsp27 or Hsp47 proteins.

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse, Cow, Rat.

Produktmerkmale: Synonyms: CRYAA, CRYA1, HSPB4, Heat shock protein beta-4

Aufreinigung: Protein G affinity chroamtography

Immunogen: Native alpha crystallin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1/2000. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Andere Bezeichnung: alpha-Crystallin A Chain

Hintergrund: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alphacrystallins possess structural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).Synonyms: CRYA1, CRYAA, HSPB4, Heat shock protein beta-4

Gen-ID: 1409

UniProt: P02489

Pathways: M Phase