RFP Antikörper (Red Fluorescent Protein) (Atto 488)

Details for Product anti-RFP Antibody No. ABIN4368319, Anbieter: Anmelden zum Anzeigen
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Reaktivität
Discosoma
81
50
37
9
7
1
1
Wirt
Camelid (Camelidae)
94
38
13
9
1
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser RFP Antikörper ist konjugiert mit Atto 488
9
7
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF)
133
68
59
52
32
27
6
5
4
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck FluoTag-Q series - high affinity monovalent binders designed for quantitative imaging
Marke FluoTag-Q
Immunogen RFP
Klon 2B12
Fragment single-domain Antibody (sdAb)
Spezifität Most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato
Kreuzreaktivität (Details) Does not cross-react with GFP, mTagBFP or mTagRFP or their most common derivatives
Produktmerkmale FluoTag-Q anti-RFP is derived from an in-house developed single-domain antibody (sdAb) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95 % guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Andere Bezeichnung RFP (RFP Antibody Abstract)
Hintergrund Red fluorescent protein RFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse RFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of RFP fusion proteins can be exceeded by far.
Molekulargewicht 26 kDa
Applikationshinweise 1:1000 (IF, ICC)
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Protect from light!
Lagerung -20 °C
Informationen zur Lagerung Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) antibody (Atto 488) (ABIN4368319) 3T3 cells transfected with the mCherry-tubulin (A, false color illustration in green)...
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