Rekombinanter GFP Antikörper (Atto 488)

Produktnummer ABIN4368303

Dieses Alpaca Monoklonal-Antikörper erkennt spezifisch GFP in IF, ICC und FACS. Er zeigt eine Reaktivität gegenüber Aequorea victoria.

Kurzübersicht für Rekombinanter GFP Antikörper (Atto 488) (ABIN4368303)

Target: GFP (Green Fluorescent Protein (GFP))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Aequorea victoria

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit Atto 488

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 1H1-1B2

Produktdetails anti-GFP Antikörper (Atto 488)

Expressionssystem: E.coli

Verwendungszweck: Camelid sdAb anti-GFP conjugated with Atto488, Clones 1H1/1B2

Spezifität: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Kreuzreaktivität (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: Produced in: E.coli

Immunogen: GFP

Labeling Ratio: A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Konzentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handhabung: Protect from light!

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Molekulargewicht: 27 kDa