Rekombinanter GFP Antikörper (Atto 488)

Produktnummer ABIN4368287

Dieses Anti-GFP-Antikörper ist ein Alpaca Monoklonal-Antikörper zur Detektion von GFP in IF, ICC und FACS. Geeignet für Aequorea victoria.

Kurzübersicht für Rekombinanter GFP Antikörper (Atto 488) (ABIN4368287)

Target: GFP (Green Fluorescent Protein (GFP))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Aequorea victoria

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit Atto 488

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 1H1

Produktdetails anti-GFP Antikörper (Atto 488)

Expressionssystem: E.coli

Verwendungszweck: Camelid sdAb anti-GFP conjugated with Atto488, Clones 1H1

Spezifität: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Kreuzreaktivität (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: Produced in: E.coli

Immunogen: GFP

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Konzentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handhabung: Protect from light!

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Molekulargewicht: 27 kDa