Cyclin B1 Antikörper (Ser147)

Produktnummer ABIN401648

Der Kaninchen Polyklonal Anti-Cyclin B1-Antikörper wurde für WB, IF und IHC (p) validiert. Er ist geeignet, Cyclin B1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Cyclin B1 Antikörper (Ser147) (ABIN401648)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cyclin B1 Antikörper

Bindungsspezifität: Ser147

Spezifität: Cyclin B1 Antibody detects endogenous levels of total Cyclin B1 protein.

Aufreinigung: Affinity chromatography

Immunogen: Synthesized non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Serine 147 (A-F-SP-D-V)

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: 1/50 - 1/100. Immunofluorescence: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1

Hintergrund: In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.Synonyms: CCNB, CCNB1, Cyclin-B1, G2/mitotic-specific cyclin-B1

Gen-ID: 891

NCBI Accession: NP_114172

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase