GATA3 Antikörper (N-Term)

Produktnummer ABIN401530

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch GATA3 in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Schwein und Hund und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für GATA3 Antikörper (N-Term) (ABIN401530)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GATA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-GATA3 Antikörper

Bindungsspezifität: N-Term

Sequenz: EVTADQPRWV SHH-C

Spezifität: This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Referenzen für anti-GATA3 Antikörper (ABIN401530)

Lee, Bae, Seo, Cho, Bae, Oh, Park: "Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).

Seo, Bang, Kim, Cho, Park: "Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1221-8, (2016) (PubMed).

Bang, Seo, Seo, Jo, Jung, Yu, Park, Park: "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117524, (2015) (PubMed).

Details zu GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Andere Bezeichnung: GATA3

Hintergrund: The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

Gen-ID: 2625

NCBI Accession: NP_001002295

UniProt: P23771

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation