Mre11 Antikörper (AA 68-706)

Produktnummer ABIN401319

Dieser Anti-Mre11 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von Mre11 in WB und EIA. Geeignet für Human, Ratte, Maus, Hund und Makake.

Kurzübersicht für Mre11 Antikörper (AA 68-706) (ABIN401319)

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reaktivität: Human, Ratte, Maus, Hund, Makake

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Mre11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Mre11 Antikörper

Bindungsspezifität: AA 68-706

Spezifität: This antibody is directed against Mre11 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Macaque, Human, Canine.
Species reactivity (tested):Mouse.

Aufreinigung: Affinity Purified

Immunogen: Recombinant protein corres-ponding to amino acids 68-706 of mouse Mre11 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA: 1/10000 - 1/50000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.05 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Details zu Mre11

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Andere Bezeichnung: MRE11

Hintergrund: Mre11 is a component of the MRN complex (Mre11/Rad50/Nbs1), which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses singlestrand endonuclease activity and doublestrand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Mre11 is a nuclear protein that is widely expressed and localizes to discrete nuclear foci after treatment with genotoxic agents (including irradiation). Alternative splice isoforms are recorded for this protein.Synonyms: Double-strand break repair protein MRE11A, HNGS1, MRE11 homolog 1, MRE11 meiotic recombination 11 homolog A, MRE11A

Gen-ID: 17535

NCBI Accession: NP_061206

UniProt: Q61216

Pathways: DNA Reparatur