EGFR Antikörper (pTyr1016)

Produktnummer ABIN400878

Dieses Anti-EGFR-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von EGFR in WB, FACS und IP. Geeignet für Human.

Kurzübersicht für EGFR Antikörper (pTyr1016) (ABIN400878)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: EM-12

Produktdetails anti-EGFR Antikörper

Bindungsspezifität: pTyr1016

Spezifität: The antibody EM-12 reacts with human EGFR (ErbB1 / HER1) phosphorylated on tyrosine 1016.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Produktmerkmale: Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1, Receptor tyrosine-proteinkinase erbB-1, Proto-oncogene c-ErbB-1

Aufreinigung: Protein A affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Immunogen: Synthetic phospho-peptide covering sequence around tyrosine 1016 of human EGFR

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry. Immunoprecipitation. Western blot: 1 μg/mL. Positive control: EGF-stimulated A431. Negative control: Non-stimulated A431.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR / ERBB1

Hintergrund: The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.Synonyms: EGF Receptor, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, c-ErbB-1, erbB-1

Gen-ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine