STAT5A Antikörper (Tyr694)

Produktnummer ABIN400812

Der Maus Monoklonal anti-STAT5A Antikörper (Klon 5F6-F1) (ABIN400812) detektiert spezifisch STAT5A in WB, IF, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für STAT5A Antikörper (Tyr694) (ABIN400812)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser STAT5A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Klon: 5F6-F1

Produktdetails anti-STAT5A Antikörper

Bindungsspezifität: Tyr694

Spezifität: This phospho-specific monoclonal antibody reacts with Stat5a pTyr694 and shows minimal reactivity by ELISA against the nonphosphorylated form of the immunizing peptide.

Kreuzreaktivität (Details): Species reactivity (expected):Human and Rat (100 %).
Species reactivity (tested):Mouse.

Produktmerkmale: Synonyms: STAT-5, STAT-5A, Mammary gland factor, MGF, Signal transducer and activator oftranscription 5A

Aufreinigung: Protein A Chromatography from concentrated tissue culture supernate.

Immunogen: Synthetic peptide corresponding to residues surrounding Tyr694 of Mouse STAT5a protein.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: 1/20,000. Western blot: 1/500-1/2,000 (Expect a band approximately 91 kDa in size corresponding tophosphorylated Stat5α protein in the appropriate cell lysate or extract). Immunohistochemistry (20-40 μg/mL). Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. For low volume of reagent (25 μl), to minimizeloss of volume dilute 1/10 by adding 225 μl of the buffer stated abovedirectly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at thebottom of the vial. Use this intermediate dilution when calculating final dilutions asrecommended below. Store the vial at -20 °C or below after dilution. Avoid cycles of freezing and thawing.
Shelf life: One year from despatch (3 months from date of opening).

Haltbarkeit: 12 months

Details zu STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Andere Bezeichnung: STAT5 / STAT5A

Hintergrund: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5alpha and Stat5beta. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.Synonyms: MGF, Mammary gland factor, STAT-5, STAT-5A, Signal transducer and activator of transcription 5A

Gen-ID: 20850

UniProt: P42230

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation