PFKFB3 Antikörper (C-Term)

Produktnummer ABIN392768

Der Kaninchen Polyklonal Anti-PFKFB3-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, PFKFB3 in Proben von Human zu detektieren. Es sind 7+ Publikationen verfügbar.

Kurzübersicht für PFKFB3 Antikörper (C-Term) (ABIN392768)

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PFKFB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB04030

Produktdetails anti-PFKFB3 Antikörper

Bindungsspezifität: AA 454-484, C-Term

Homologie: Rat

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PFKFB3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 454-484 amino acids from the C-terminal region of human PFKFB3.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-PFKFB3 Antikörper (ABIN392768)

Heydasch, Kessler, Warnke, Eschrich, Scholz, Bigl: "Functional diversity of PFKFB3 splice variants in glioblastomas." in: PloS one, Vol. 16, Issue 7, pp. e0241092, (2021) (PubMed).

Lee, Lee, Yun, Jang, Kang, Kim, Choi, Park: "Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species." in: International journal of nanomedicine, Vol. 11, pp. 55-68, (2016) (PubMed).

Reddy, Fernandes, Deshpande, Weisberg, Inguilizian, Abdel-Wahab, Kung, Levine, Griffin, Sattler: "The JAK2V617F oncogene requires expression of inducible phosphofructokinase/fructose-bisphosphatase 3 for cell growth and increased metabolic activity." in: Leukemia, Vol. 26, Issue 3, pp. 481-9, (2012) (PubMed).

Ando, Uehara, Kogure, Asano, Nakajima, Abe, Kawauchi, Tanaka: "Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3." in: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi, Vol. 77, Issue 2, pp. 97-105, (2010) (PubMed).

Yamasaki, Hayashi, Okamoto, Osanai, Lee: "Insulin-independent promotion of chemically induced hepatocellular tumor development in genetically diabetic mice." in: Cancer science, Vol. 101, Issue 1, pp. 65-72, (2010) (PubMed).

Clem, Telang, Clem, Yalcin, Meier, Simmons, Rasku, Arumugam, Dean, Eaton, Lane, Trent, Chesney: "Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth." in: Molecular cancer therapeutics, Vol. 7, Issue 1, pp. 110-20, (2008) (PubMed).

Telang, Yalcin, Clem, Bucala, Lane, Eaton, Chesney: "Ras transformation requires metabolic control by 6-phosphofructo-2-kinase." in: Oncogene, Vol. 25, Issue 55, pp. 7225-34, (2006) (PubMed).

Details zu PFKFB3

Target: PFKFB3 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 3 (PFKFB3))

Andere Bezeichnung: PFKFB3

Hintergrund: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Molekulargewicht: 59609

Gen-ID: 5209

NCBI Accession: NP_001138915, NP_004557

UniProt: Q16875

Pathways: AMPK Signaling, Regulation of Carbohydrate Metabolic Process