SMAD3 Antikörper (pSer208)

Produktnummer ABIN389656

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch SMAD3 in IF und DB. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.

Kurzübersicht für SMAD3 Antikörper (pSer208) (ABIN389656)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Dot Blot (DB)

Klon: RB31592-RB31594

Produktdetails anti-SMAD3 Antikörper

Bindungsspezifität: pSer208

Homologie: C, M, Pig, Rat

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S208 of human SMAD3.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:200. IF: 1:10~50. DB: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Haltbarkeit: 6 months

Referenzen für anti-SMAD3 Antikörper (ABIN389656)

Cohen-Solal, Merrigan, Chan, Goydos, Chen, Foran, Liu, Lasfar, Reiss: "Constitutive Smad linker phosphorylation in melanoma: a mechanism of resistance to transforming growth factor-?-mediated growth inhibition." in: Pigment cell & melanoma research, Vol. 24, Issue 3, pp. 512-24, (2011) (PubMed).

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: SMAD3

Hintergrund: SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80 % of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

Molekulargewicht: 48081

Gen-ID: 4088

NCBI Accession: NP_001138574, NP_001138575, NP_001138576, NP_005893

UniProt: P84022

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie