NFkB Antikörper (pSer536)

Produktnummer ABIN389601

Zitiert in 2+ Publikationen. Der Kaninchen Polyklonal anti-NFkB Antikörper (Klon RB5212) (ABIN389601) detektiert spezifisch NFkB in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für NFkB Antikörper (pSer536) (ABIN389601)

Target: NFkB (Nuclear Factor kappa B (NFkB))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NFkB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: RB5212

Produktdetails anti-NFkB Antikörper

Bindungsspezifität: pSer536

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This NFKB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NFKB.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-NFkB Antikörper (ABIN389601)

Li, Lu, Yu, Han, Wang, Mao, Shen, Wang, Tang, Li, Song: "miR-221/222 promote cancer stem-like cell properties and tumor growth of breast cancer via targeting PTEN and sustained Akt/NF-κB/COX-2 activation." in: Chemico-biological interactions, Vol. 277, pp. 33-42, (2017) (PubMed).

Wang, Cai, Shao, Siddiqui, Palanisamy, Li, Ren, Ayala, Ittmann: "Activation of NF-{kappa}B by TMPRSS2/ERG Fusion Isoforms through Toll-Like Receptor-4." in: Cancer research, Vol. 71, Issue 4, pp. 1325-33, (2011) (PubMed).

Details zu NFkB

Target: NFkB (Nuclear Factor kappa B (NFkB))

Andere Bezeichnung: NFKB

Hintergrund: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Molekulargewicht: 60219

Gen-ID: 5970

NCBI Accession: NP_001138610, NP_001230913, NP_068810

UniProt: Q04206

Pathways: Ubiquitin Proteasome Pathway, S100 Proteine