BMI1 Antikörper (C-Term)

Produktnummer ABIN389084

Der Kaninchen Polyklonal Anti-BMI1-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, BMI1 in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für BMI1 Antikörper (C-Term) (ABIN389084)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BMI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB02926

Produktdetails anti-BMI1 Antikörper

Bindungsspezifität: AA 257-286, C-Term

Homologie: B, C, M

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This BMI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human BMI1.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-BMI1 Antikörper (ABIN389084)

Wang, Venugopal, Manoranjan, McFarlane, OFarrell, Nolte, Gunnarsson, Hollenberg, Kwiecien, Northcott, Taylor, Hawkins, Singh: "Sonic hedgehog regulates Bmi1 in human medulloblastoma brain tumor-initiating cells." in: Oncogene, Vol. 31, Issue 2, pp. 187-99, (2012) (PubMed).

Lewis, Segditsas, Deheragoda, Pollard, Jeffery, Nye, Lockstone, Davis, Clark, Stamp, Poulsom, Wright, Tomlinson: "Severe polyposis in Apc(1322T) mice is associated with submaximal Wnt signalling and increased expression of the stem cell marker Lgr5." in: Gut, Vol. 59, Issue 12, pp. 1680-6, (2010) (PubMed).

Sangiorgi, Capecchi: "Bmi1 lineage tracing identifies a self-renewing pancreatic acinar cell subpopulation capable of maintaining pancreatic organ homeostasis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 17, pp. 7101-6, (2009) (PubMed).

Details zu BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Andere Bezeichnung: BMI1

Hintergrund: BMI is a component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2. BMI cooperates with the MYC oncogene to produce B-lymphomas.

Molekulargewicht: 36949

Gen-ID: 648

NCBI Accession: NP_001190991, NP_005171

UniProt: P35226

Pathways: Zellzyklus, Autophagie