ATG7 Antikörper (AA 494-523)

Produktnummer ABIN388522

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ATG7 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human und Maus und wurde in 10+ Publikationen erwähnt.

Kurzübersicht für ATG7 Antikörper (AA 494-523) (ABIN388522)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB7472

Produktdetails anti-ATG7 Antikörper

Bindungsspezifität: AA 494-523

Homologie: Rat

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 494-523 amino acids from human ATG7.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. IHC-P: 1:25

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-ATG7 Antikörper (ABIN388522)

Li, Zhou, Yang, Li, Zhang, Zheng: "Curcumin induces apoptotic cell death and protective autophagy in human gastric cancer cells." in: Oncology reports, Vol. 37, Issue 6, pp. 3459-3466, (2018) (PubMed).

Oikonomou, Moretti, Renga, Galosi, Borghi, Pariano, Puccetti, Palmerini, Amico, Carotti, Prezioso, Spolzino, Finocchi, Rossi, Velardi, Aversa, Napolioni, Romani: "Noncanonical Fungal Autophagy Inhibits Inflammation in Response to IFN-γ via DAPK1." in: Cell host & microbe, Vol. 20, Issue 6, pp. 744-757, (2017) (PubMed).

Gao, Li, Ding, Qi, Yang: "Cepharanthine Induces Autophagy, Apoptosis and Cell Cycle Arrest in Breast Cancer Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 41, Issue 4, pp. 1633-1648, (2017) (PubMed).

Laggner, Pollreisz, Schmidinger, Schmidt-Erfurth, Chen: "Autophagy mediates cell cycle response by regulating nucleocytoplasmic transport of PAX6 in limbal stem cells under ultraviolet-A stress." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180868, (2017) (PubMed).

Okamoto, Sakimoto, Imai, Senoo, Shidoji: "Induction of an incomplete autophagic response by cancer-preventive geranylgeranoic acid (GGA) in a human hepatoma-derived cell line." in: The Biochemical journal, Vol. 440, Issue 1, pp. 63-71, (2011) (PubMed).

Wei, Kulp, Chen: "Energy restriction as an antitumor target of thiazolidinediones." in: The Journal of biological chemistry, Vol. 285, Issue 13, pp. 9780-91, (2010) (PubMed).

Yang, Liu, Goga, Kim, Yuneva, Bishop: "Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 31, pp. 13836-41, (2010) (PubMed).

Bigford, Alonso, Dietrich, Keane: "A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury." in: Journal of neurotrauma, Vol. 26, Issue 5, pp. 703-20, (2009) (PubMed).

Ding, Shi, Zhou, Qiu, Xu, Dai, Shi, Wang, Ke, Wu, Fan: "Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma." in: Cancer research, Vol. 68, Issue 22, pp. 9167-75, (2008) (PubMed).

Xu, Liang, Haridas, Gaikwad, Connolly, Mills, Gutterman: "A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase." in: Cell death and differentiation, Vol. 14, Issue 11, pp. 1948-57, (2007) (PubMed).

Details zu ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Andere Bezeichnung: ATG7

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Molekulargewicht: 77960

Gen-ID: 10533

NCBI Accession: NP_001129503, NP_001138384, NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagie