ATG7 Antikörper (N-Term)

Produktnummer ABIN388520

Der Kaninchen Polyklonal Anti-ATG7-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, ATG7 in Proben von Human und Maus zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für ATG7 Antikörper (N-Term) (ABIN388520)

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: RB7467

Produktdetails anti-ATG7 Antikörper

Bindungsspezifität: AA 22-51, N-Term

Homologie: Rat

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human ATG7.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit: 6 months

Referenzen für anti-ATG7 Antikörper (ABIN388520)

Wang, Hu, Sun, Zhuo, He, Wang, Yan, Liu, Luan, Dai, Yang, Huang, Zhou, Zhang, Zhai: "Down-regulation of Risa improves insulin sensitivity by enhancing autophagy." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 9, pp. 3133-45, (2017) (PubMed).

Yang, Zhao, Mei, Jiang, Geng, Li, Yao, Liu, Kong, Cao: "HMGA2 plays an important role in Cr (VI)-induced autophagy." in: International journal of cancer, Vol. 141, Issue 5, pp. 986-997, (2017) (PubMed).

Chiu, Kulp, Soni, Wang, Gunn, Schlesinger, Chen: "Eradication of intracellular Salmonella enterica serovar Typhimurium with a small-molecule, host cell-directed agent." in: Antimicrobial agents and chemotherapy, Vol. 53, Issue 12, pp. 5236-44, (2009) (PubMed).

Details zu ATG7

Target: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Andere Bezeichnung: ATG7

Hintergrund: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Molekulargewicht: 77960

Gen-ID: 10533

NCBI Accession: NP_001129503, NP_001138384, NP_006386

UniProt: O95352

Pathways: Response to Water Deprivation, Autophagie