DYKDDDDK Tag Antikörper (FITC)

Produktnummer ABIN387724

Produktdetails anti-DYKDDDDK Tag Antikörper (FITC)

Target: DYKDDDDK Tag

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist konjugiert mit FITC

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Marke: THE™

Sequenz: DYKDDDDK

Spezifität: THE^TM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.

Produktmerkmale: This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Aufreinigung: Protein A affinity column

Immunogen: A synthetic peptide DYKDDDDK coupled - KLH

Klon: 5A8E5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/mL
Flow cytometry: 0.5-2 µg for 1 x 10^6 cells
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-DYKDDDDK Tag Antikörper (FITC) (ABIN387724)

Pumroy, Nardozzi, Hart, Root, Cingolani: "Nucleoporin Nup50 stabilizes closed conformation of armadillo repeat 10 in importin ?5." in: The Journal of biological chemistry, Vol. 287, Issue 3, pp. 2022-31, (2012) (PubMed).

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Abstract: DYKDDDDK Tag Produkte

Substanzklasse: Tag