PDE11A Antikörper (Internal Region)

Produktnummer ABIN374774

Der Ziege Polyklonal anti-PDE11A Antikörper wird verwendet zum Nachweis von PDE11A in Proben von Human, Maus, Ratte und Hund. Er wurde validiert für WB.

Kurzübersicht für PDE11A Antikörper (Internal Region) (ABIN374774)

Target: PDE11A (phosphodiesterase 11A (PDE11A))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PDE11A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PDE11A Antikörper

Bindungsspezifität: Internal Region

Spezifität: This antibody reacts to PDE11A.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Immunogen: Peptide with sequence C-NSFKESMEKSSYSD, from the internal region of the protein sequence

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/2000. Western Blot: 0.5 - 1.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PDE11A

Target: PDE11A (phosphodiesterase 11A (PDE11A))

Andere Bezeichnung: PDE11A

Hintergrund: One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is Phosphodiesterase type 11. The human PDE11A contains an open reading frame encoding a 490 amino acid protein (55-56 kDa). The PDE11A has an N terminus GAF domain homologous to other signaling molecule as found in PDE2, PDE5, PDE6, PDE10, which potentially represents an allosteric cGMP and other small signaling molecules. PDE11A hydrolyzes both cGMP and cAMP, it is sensitive to non selective PDE inhibitor IBMX as well as zaprinast and dipyrimadole inhibitors that are generally selective for cGMP specific enzymes. PDE11A expression is diverse and is found in high levels in skeletal muscle, prostate, kidney, liver, pituitary and testis. There are several isoforms known: Isoform 1 is present in prostate, pituitary, heart and liverbut not in testis or in penis. Isoform 2 is believed to be expressed in testis and Isoform 4 in the adrenal cortex.Synonyms: 5'-cyclic-AMP and -GMP phosphodiesterase 11A, Dual 3', PDE11A1, PDE11A2, PDE11A3, PDE11A4, Phosphodiesterase 11A, cAMP and cGMP phosphodiesterase 11A

Gen-ID: 50940

NCBI Accession: NP_001070664

UniProt: Q9HCR9

Pathways: cAMP Metabolic Process