14-3-3 sigma/SFN Antikörper (Internal Region, N-Term)

Produktnummer ABIN374638

Dieser Ziege Polyklonal Antikörper detektiert spezifisch 14-3-3 sigma/SFN in WB und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Hund.

Kurzübersicht für 14-3-3 sigma/SFN Antikörper (Internal Region, N-Term) (ABIN374638)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser 14-3-3 sigma/SFN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-14-3-3 sigma/SFN Antikörper

Bindungsspezifität: Internal Region, N-Term

Sequenz: C-DLHTLSEDSY KDST

Spezifität: This antibody recognizes 4-3-3 sigma/Stratifin.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Porcine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_006133.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western Blot: 0.1-0.3 μg/mL. Approx.28 kDa band observed in Human Skin lysates (calculated MW of 27.8 kDa accordingto NP_006133.1). Immunohistochemistry on Paraffin Sections: 2-3 μg/mL. In paraffin embedded Human Skin, this antibody shows strong nuclear staining in selectedcells of the basal layer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Andere Bezeichnung: 14-3-3 Protein sigma / SFN

Hintergrund: Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. As 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. 14-3-3 sigma may be required for Raf-1 kinase activity and phosphorylation, among many other functions.Synonyms: Epithelial cell marker protein 1, HME1, Stratifin

Gen-ID: 2810, 9606

UniProt: P31947

Pathways: p53 Signalweg, Myometrial Relaxation and Contraction