MPG Antikörper (C-Term)

Produktnummer ABIN374423

Der Ziege Polyklonal anti-MPG Antikörper wird verwendet zum Nachweis von MPG in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für MPG Antikörper (C-Term) (ABIN374423)

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MPG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MPG Antikörper

Bindungsspezifität: C-Term

Sequenz: C-SVVDRVAEQD TQA

Spezifität: This antibody detects MPG / ANPG at C-term.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002425.2, NP_001015052.1, NP_001015054.1. Genename: MPG

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 16000. Western blot: 1-3 μg/mL. Approx 28-30 kDa band observed in lysates of cell line K562(calculated MW of 30.0 kDa according to NP_001015054.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Details zu MPG

Target: MPG (N-Methylpurine-DNA Glycosylase (MPG))

Andere Bezeichnung: MPG / ANPG

Hintergrund: MPG (N-methylpurine DNA glycosylase, or 3-methyladenine (3MeA) DNA glycosylase) repairs the 3MeA lethal lesion blocking DNA replication in Escherichia coli. Analysis of human cDNA libraries found a cDNA that would hybridize to human genomic DNA but not to E. coli or yeast DNA. This MPG gene has been mapped to human chromosome 16 by analysis of a panel of DNAs from mouse/human and hamster/human hybrid cell lines.Synonyms: 3-alkyladenine DNA glycosylase, 3-methyladenine DNA glycosidase, AAG, ADPG, DNA-3-methyladenine glycosylase, MID1, N-methylpurine-DNA glycosylase

Gen-ID: 4350

NCBI Accession: NP_002425

UniProt: P29372

Pathways: DNA Reparatur