FOXA1 Antikörper (C-Term)

Produktnummer ABIN374334

Dieses Anti-FOXA1-Antikörper ist ein Ziege Polyklonal-Antikörper zur Detektion von FOXA1 in WB, IHC (p), ChIP und EIA. Geeignet für Human, Ratte, Maus, Rind (Kuh) und Schwein.

Kurzübersicht für FOXA1 Antikörper (C-Term) (ABIN374334)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser FOXA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Produktdetails anti-FOXA1 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-GVYSRPVLNT S

Spezifität: This antibody detects FOXA1.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Pig, Cow.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_004487.2 Genename: FOXA1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1/16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in Human lysates of cell lineHepG2 (calculated MW of 49.0 kDa according to NP_004487.2). An additional band ofunknown identity was also consistently observed at 30 kDa. This band was successfullyblocked by incubation with the immunising peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Anonymous customers used this product successfully in ChIP with MCF7.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Andere Bezeichnung: FOXA1 / TCF3A

Hintergrund: FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.Synonyms: Forkhead box protein A1, HNF-3-alpha, HNF-3A, HNF3A, Hepatocyte nuclear factor 3-alpha, TCF-3A, Transcription factor 3A

Molekulargewicht: 49.1 kDa (NP_004487.2)

Gen-ID: 3169

NCBI Accession: NP_004487

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis