IRF5 Antikörper (C-Term)

Produktnummer ABIN374255

Der Ziege Polyklonal anti-IRF5 Antikörper wird verwendet zum Nachweis von IRF5 in Proben von Human, Maus, Ratte und Rind (Kuh). Er wurde validiert für WB, IP, IHC (p), ChIP und EIA.

Kurzübersicht für IRF5 Antikörper (C-Term) (ABIN374255)

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IRF5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Produktdetails anti-IRF5 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-QGPWPMHPAG MQ

Spezifität: This antibody is expected to recognize all four reported isoforms.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1. Genename: IRF5

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.1-0.3 μg/mL. Approx 57 kDa band observed in lysates of cell line A549. Intransfected HEK293 transiently expressing IRF5 a strong band of approx.67 kDa isobserved. This band is not observed in the non-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Details zu IRF5

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Andere Bezeichnung: IRF5

Hintergrund: Interferon regulatory factors (IRFs) are transcription factors that possess a novel helix turn helix DNA binding motif and mediate virus and interferon (IFN) induced signaling pathways. The IRF5 protein is structurally related to IRF6. Like other IRFs, IRF5 and IRF6 contain 5 conserved tryptophan residues in the 115 amino acid N terminal DNA binding domain. IRF5 has a central IRF association domain (IAD) and, like IRF7 and IRF8, is induced by IFNA/IFNB stimulation. IRF5 has a type I IFN transactivation potential similar to that of IRF1. IRF3 and IRF5 form heterodimers and are both required for effective induction of IFNA gene transcription.Synonyms: IRF-5, Interferon regulatory factor 5

Gen-ID: 3663

NCBI Accession: NP_002191

UniProt: Q13568

Pathways: TLR Signalweg, Autophagie