APEX1 Antikörper (N-Term)

Produktnummer ABIN374178

Der Ziege Polyklonal anti-APEX1 Antikörper (ABIN374178) detektiert spezifisch APEX1 in WB, IHC (p) und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Rind (Kuh), Schwein und Hund.

Kurzübersicht für APEX1 Antikörper (N-Term) (ABIN374178)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reaktivität: Human, Rind (Kuh), Schwein, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser APEX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-APEX1 Antikörper

Bindungsspezifität: N-Term

Sequenz: PKRGKKGAVA EDGD-C

Spezifität: This antibody detects APEX1 / REF-1 at N-term.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic peptide from the N-Terminus of Human APEX1 / APE1 (NP_001632.2). Genename: APEX1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 35 kDa band observed in Human epidermoid fibroblastA431 and Human cervix epitheloid fibroblast HeLa lysates (calculated MW of 35.5 kDaaccording to NP_001632.2, NP_542379.1, NP_542380.1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Andere Bezeichnung: APEX1 / REF-1

Hintergrund: Apurinic / apyrimidinic endonuclease (APE1 or REF1) is responsible for the incision of DNA basic sites during base excision repair. It has also been shown to stimulate the DNA binding activity of numerous transcription factors that are involved in cancer promotion and progression. Alterations in APE1 levels have been described in some cancers. APE1 is part of the cellular response to oxidative stress and protects cells from the genotoxic and cytotoxic effect of oxidizing agents.Synonyms: APE, APEN, APEX, APEX nuclease, APX, Apurinic-apyrimidinic endonuclease 1, HAP1, Protein REF-1, REF1

Gen-ID: 328

NCBI Accession: NP_001632

UniProt: P27695

Pathways: DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus