BAK1 Antikörper (N-Term)

Produktnummer ABIN374174

Es gibt 1 Publikation für dieses Produkt. Der Ziege Polyklonal anti-BAK1 Antikörper wird verwendet zum Nachweis von BAK1 in Proben von Human, Maus, Ratte und Schwein. Er wurde validiert für WB und EIA.

Kurzübersicht für BAK1 Antikörper (N-Term) (ABIN374174)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-BAK1 Antikörper

Bindungsspezifität: N-Term

Sequenz: ASGQGPGPPR QE-C

Spezifität: This antibody detects Bcl-2-like 7 at N-term.

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_001179.1 Genename: BAK1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit dilution 1: 32000. Western Blot: 0.5-1 μg/mL. Approx 25 kDa band seen in A431 cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Referenzen für anti-BAK1 Antikörper (ABIN374174)

Huang, Li, Pang, Chen, Xiang, Feng, Su, Li, Zhang, Liu: "MicroRNA-29c Correlates with Neuroprotection Induced by FNS by Targeting Both Birc2 and Bak1 in Rat Brain after Stroke." in: CNS neuroscience & therapeutics, Vol. 21, Issue 6, pp. 496-503, (2016) (PubMed).

Details zu BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Andere Bezeichnung: Bcl-2-Like 7

Hintergrund: Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.Synonyms: Apoptosis regulator BAK, BAK, BAK1, BCL2L7, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, Bcl2-L-7, CDN1, cell death inhibitor 1

Gen-ID: 578

NCBI Accession: NP_001179

UniProt: Q16611

Pathways: Apoptose, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response