SOCS3 Antikörper (C-Term)

Produktnummer ABIN374066

Der Ziege Polyklonal anti-SOCS3 Antikörper wird verwendet zum Nachweis von SOCS3 in Proben von Human, Maus, Ratte, Rind (Kuh), Schwein und Hund. Er wurde validiert für WB.

Kurzübersicht für SOCS3 Antikörper (C-Term) (ABIN374066)

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SOCS3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-SOCS3 Antikörper

Bindungsspezifität: C-Term

Sequenz: C-PIREFLDQYD APL

Spezifität: This antibody detects SOCS3 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, canine (Dog), Pig, Cow.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_003946.3. Genename: SOCS3

Anwendungsinformationen

Applikationshinweise: ELISA: Limit dilution: 1/16000. Western Blot: 4-6 μg/mL. Approx 24 kDa band observed in MOLT-4 lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu SOCS3

Target: SOCS3 (Suppressor of Cytokine Signaling 3 (SOCS3))

Andere Bezeichnung: SOCS3 / CIS3

Hintergrund: Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS-1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT-induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways.Synonyms: CIS-3, Cytokine-inducible SH2 protein 3, SOCS-3, SSI-3, SSI3, STAT-induced STAT inhibitor 3, Suppressor of cytokine signaling 3

Gen-ID: 9021

NCBI Accession: NP_003946

UniProt: O14543

Pathways: JAK-STAT Signalweg, Response to Growth Hormone Stimulus, Hepatitis C