PLK1 Antikörper (pThr210)

Produktnummer ABIN372703

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PLK1 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Xenopus laevis, Rind (Kuh), Hund, Zebrafisch (Danio rerio) und Non-Human Primate.

Kurzübersicht für PLK1 Antikörper (pThr210) (ABIN372703)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reaktivität: Human, Maus, Ratte, Xenopus laevis, Rind (Kuh), Hund, Zebrafisch (Danio rerio), Non-Human Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PLK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PLK1 Antikörper

Bindungsspezifität: pThr210

Spezifität: This antibody recognizes the ~66k PLK protein phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Canine, Mouse, Xenopus, Zebrafish and non-Human Primates.
Species reactivity (tested):Rat and Human.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr210 of PLK1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Details zu PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1

Hintergrund: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK's is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).Synonyms: PLK-1, Polo-like kinase 1, STPK13, Serine/threonine-protein kinase PLK1

Gen-ID: 5347

NCBI Accession: NP_005021

UniProt: P53350

Pathways: Zellzyklus, M Phase