PAK1 Antikörper (pThr402)

Produktnummer ABIN372696

Produktdetails anti-PAK1 Antikörper

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Bindungsspezifität: pThr402

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: This antibody recognizes the ~68k to ~70k PAK protein phosphorylated at Thr402. The immunolabeling of PAK is completely eliminated by lambda-phosphatase treatment.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Bovine, Canine and Mouse.
Species reactivity (tested):Rat.

Aufreinigung: Sequential Chromatography on phospho- and dephosphopeptide affinity columns.

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr402 of Rat p21 activated kinase 2 (PAK-2).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at-20 °C.

Referenzen für anti-PAK1 Antikörper (ABIN372696)

Nie, Lilley, Pan, Faruque, Liu, Zhang, Sanes, Han, Shi: "SAD-A potentiates glucose-stimulated insulin secretion as a mediator of glucagon-like peptide 1 response in pancreatic ? cells." in: Molecular and cellular biology, Vol. 33, Issue 13, pp. 2527-34, (2013) (PubMed).

Details zu PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Andere Bezeichnung: PAK1 (PAK1 Produkte)

Synonyme: X-PAK1 antikoerper, XPak1 antikoerper, pak antikoerper, pak-1 antikoerper, pakalpha antikoerper, PAKalpha antikoerper, AW045634 antikoerper, PAK-1 antikoerper, Paka antikoerper, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antikoerper, p21 (RAC1) activated kinase 1 antikoerper, p21 protein (Cdc42/Rac)-activated kinase 1 antikoerper, Serine/threonine-protein kinase pak-1 antikoerper, pak1.S antikoerper, PAK1 antikoerper, Pak1 antikoerper, pak-1 antikoerper

Hintergrund: In mammals, there are several identified isoforms of p21-activated protein kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003, Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).Synonyms: Alpha-PAK, Beta-PAK, Gamma-PAK, OPHN3, Oligophrenin-3, PAK 1, PAK 2, PAK 3, PAK alpha, PAK beta, PAK gamma, PAK-1, PAK-2, PAK-3, PAK65, Serine/threonine-protein kinase PAK 1, Serine/threonine-protein kinase PAK 2, Serine/threonine-protein kinase PAK 3, p21-activated kinase 1, p21-activated kinase 2, p21-activated kinase 3, p58, p65-PAK

Gen-ID: 5058

NCBI Accession: NP_001122092

UniProt: Q13153

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis