GCLC Antikörper (AA 295-313)

Produktnummer ABIN372566

Der Kaninchen Polyklonal Anti-GCLC-Antikörper wurde für WB, IP, IHC (p) und EIA validiert. Er ist geeignet, GCLC in Proben von Human, Maus, Ratte, Rind (Kuh) und Affe zu detektieren.

Kurzübersicht für GCLC Antikörper (AA 295-313) (ABIN372566)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-GCLC Antikörper

Bindungsspezifität: AA 295-313

Spezifität: Recognizes the heavy subunit of GCS.

Kreuzreaktivität (Details): Species reactivity (expected):Human, Monkey, Mouse and Rat, Bovine (weak).
Species reactivity (tested):Rat.

Aufreinigung: Protein A Chromatography.

Immunogen: Synthetic peptide derived from corresponding to aa 295-313 of Rat GCLC (heavy subunit of GCS).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 10 mM PBS, pH 7.4 containing 0.09 % Sodium Azide as presrevtaive and 40 % Glycerol, 0.2 % BSA as stabilizers..

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (Add 40-50% glycerol) at-20 °C for longer.

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: GCLC / GLCLC

Hintergrund: Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells GSH is synthesized de novo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH synthetase. GCS catalyses the first and rate limiting step and is important in GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlle by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2 mM and increases the inhibitory constant for GSH.Synonyms: GCS heavy chain, GLCL, GLCLC, Gamma-ECS, Gamma-glutamylcysteine synthetase, Glutamate-cysteine ligase catalytic subunit

Gen-ID: 2729

NCBI Accession: NP_001184044

UniProt: P48506

Pathways: Cell RedoxHomeostasis