GCLC Antikörper (Middle Region)

Produktnummer ABIN2785780

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GCLC in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen, Meerschweinchen, Hund, Pferd und Saccharomyces cerevisiae. Es wurde in 2+ Publikationen zitiert.

Kurzübersicht für GCLC Antikörper (Middle Region) (ABIN2785780)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen, Meerschweinchen, Hund, Pferd, Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GCLC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GCLC Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: GCLC Antibody - middle region

Sequenz: RISKSRYDSI DSYLSKCGEK YNDIDLTIDK EIYEQLLQEG IDHLLAQHVA

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLC

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54577-100UG

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: prevent freeze-thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-GCLC Antikörper (ABIN2785780)

Ramani, Tomasi, Yang, Ko, Lu: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).

Bardag-Gorce, Oliva, Lin, Li, French, French: "Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease." in: Experimental and molecular pathology, Vol. 90, Issue 1, pp. 123-30, (2011) (PubMed).

Details zu GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Andere Bezeichnung: GCLC

Hintergrund:

Background Information: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Gene Name: Glutamate-cysteine ligase, catalytic subunit

Alternative Symbols: GCL, GCS, GLCL, GLCLC

Protein Name: Glutamate--cysteine ligase catalytic subunit

Molekulargewicht: 73 kDa

Gen-ID: 2729

NCBI Accession: NP_001489

UniProt: P48506

Pathways: Cell RedoxHomeostasis